README for Figure 1A+B.csv 
*** This file contains the raw data obtained on DNA and DNA-Rok complexes using tethered particle motion represented in Figure 1A and 1B of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es

Legend Figure 1: B. subtilis Rok compacts DNA. A)  Histograms of Root Mean Square displacement (RMS) obtained for 
32%GC DNA as a function of Rok at concentrations of 0, 2, 3, 4, 5, 6, 7, 9 and 10 nM as measured by TPM in the 
presence of 50 mM KCl. The histograms were fitted to Gaussian distributions, in which the RMS value at ~150 nm 
represents bare DNA and the population with an RMS at ~100 nm represents DNA bound by Rok. The bare DNA and 
Rok-DNA complex populations are highlighted with a light blue and magenta box, respectively. The data for each 
concentration originates from at least two independent measurements. B) RMS values obtained for 32%GC DNA as a 
function of Rok at concentrations from 0 nM to 10 nM.  Blue and magenta dots represent the average RMS resulting 
from fitting with a Gaussian distribution, where blue represents bare DNA and magenta Rok-DNA complexes, 
respectively. Error bars represent the propagated standard deviation from at least two independent measurements.
Due to their small size, some error bars are hidden behind the data points. How the DNA tethers are distributed 
among the two populations is not taken into account in this representation. Dashed lines are lines to guide the 
eye. c) RMS as a function of protein concentration of E. coli H-NS and HU and M. fervidus HMfA. Data were taken 
from van der Valk et al. 2017 (H-NS)1, Driessen et al. 2014 (HU)2 and Henneman et al. 2021 (HMfA)3. Error bars 
represent the standard deviation; due to their small size they are hidden behind the data points. Dashed lines 
are lines to guide the eye. 

 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for each protein concentration(s) is given in this table as plotted in figure 2A and 2B.
Column A: Rok WT(nM) = Concentration of Rok in nanomolar
Column B: Replicate = number of replicate
Column C: RMS (nm) = Root mean square deviation in nanometer measured for each bead 
Column D: rho = anisotropic ratio (movement in x-direction/movement in y-direction) 
Column E: stdev = standard deviation 
Column F: FoV = field of view (data with the same FoV were collected in one image) 
Column G: bead = beads in each FoV are represented by a unique number 
The values given in column D and E are tether quality criteria and used for data filtering as described in the associated article.